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EZ™ Luciferase Assay System new

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1 EZ™ Luciferase Assay System EZ040S - 100 rxns ₩ 99,000 추가
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Product description

EZ™ Luciferase Assay System is a product with enhanced stability and increased sensitivity compared to existing experiment method, and it is suitable for firefly luciferase reporter assay in mammalian, plant, and bacteria cell. Simply consisting of Lysis buffer, Reaction buffer, and substrate in power form, it is optimized in convenience. Once expressed in cells after transfection, Firefly luciferase is erupted from cells through the action of lysis buffer, and then it facilitates the oxidation of D-luciferin, a substrate mixed with reaction buffer. As a result, oxyluciferin is generated while light is emitted simultaneously. For compensating for the considerable deviation among samples in existing experiment method due to the rapid destabilization of generated light, this product uses coenzymes A for increasing enzyme turnover rate and accordingly enhancing the stability of light, which leads to uniform light intensity maintained for more than 1 minute.

 

Component

- Luciferase Assay Buffer

- Luciferase Assay Substrate

- 5X Luciferase Cell Lysis Buffer

 

Characteristics

- High sensitivity which can be measured up to 10-20 moles

- 5 minutes from cell lysis to assay

 

Application

- Promoter assay

- Transfection optimization




Figure 1. Comparison of luciferase activity of Enzynomics and competitor in various cells expressing firefly luciferase gene

Transfected cells were lysed with 1X Luciferase Cell Culture Lysis Buffer. The lysate was mixed with Luciferase Reaction Buffer containing substrate and analyzed for 10 seconds using a GloMax 96 luminometer. 




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